Detection and analysis of human bocavirus in children hospitalized with respiratory tract infection from 2010 to 2011 in Urumqi area

Objective To study human bocavirus (HBoV) infections in hospitalized children with respiratory tract infection in Urumqi area.Methods Nasopharyngeal aspirates (NPA) samples were collected from 525 hospitalized children with respiratory tract infection at People's Hospital of Xinjiang Uygur Autonomous Region from 2010 to 2011.Nested PCR was used to amplify NS1 fragments to screen HBoV 1 to HBoV 4.Results The overall frequency of HBoVs was 8.38％ (44/525),including six cases of HBoV 1,thirty-eight cases of HBoV 2.HBoV 3 and HBoV 4 were not detected.HBoVs positive rates of Uygur and Hui children were statistically significantly higher than Han respectively (P ＜0.05).Nucleotide sequences of six strains of HBoV 1 were 97.7％ ～ 99.4％ identical with that of 3 reference strains.Thirty-eight strains of HBoV 2 had 93.6 ～ 100％ identical nucleotide sequence with 3 reference strains.HBoVs mainly occurred in winter and spring.No statistically significant differences in sex and ages were found.Conclusions HBoV 1 and HBoV 2 were circulating among hospitalized children with respiratory tract infection from 2010 to 2011 in Urumqi area with HBoV 2 as the dominant subtype.HBoVs positive rates of Uygur and Hui children were higher than Han respectively. Key words: Human bocavirus;  Respiratory tract infection;  Children;  Nested PCR