Study on the metabolites of 1α, 25-dihydroxyvitamin D4

Abstract Three major metabolites of 1α,25-dihydroxyvitamin D 4 were isolated from the bile of rat and the structures were elucidated on the basis of spectral data and the periodate oxidative cleavage of the diol structures of the metabolites. One of the metabolites was the known calcitroic acid. Another two metabolites were isomers and identified as 9,10-secoergosta-5,7,10(19)-triene-1α,3β,24,25-tetrahydroxy-26-oic acid and 9,10-secoergosta-5,7,10(19)-triene-1α,3β,24,25-tetrahydroxy-28-oic acid. It was found that 1α,25-dihydroxyvitamin D 4 is metabolized in a similar manner in vivo to that of 1α,25-dihydroxyvitamin D 2 but differently from 1α,25-dihydroxyvitamin D 3 .