Terasakiella brassicae sp. nov., isolated from the wastewater of a pickle-processing factory, and emended descriptions of Terasakiella pusilla and the genus Terasakiella

A Gram-stain-negative, motile, polyhydroxybutyrate-accumulating, aerobic, S-shaped bacterium, designated B3T, was isolated from the wastewater of a pickle-processing factory. 16S rRNA gene sequence similarity analysis showed that it was most closely related to the type strain, Terasakiella pusilla (96.6 % similarity). Strain B3T was able to grow at 4–40 °C (optimum 32–37 °C), pH 5.5–9.0 (optimum 6.5–7.5) and with 0.5–8 % (w/v) NaCl present (optimum 1–2 %, w/v). Chemotaxonomic analysis showed that the respiratory quinone was ubiquinone Q-10, the major fatty acids included C16 : 0, C18 : 1ω7c and C16 : 1ω7c and/or iso-C15:2-OH. The major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, aminophospholipid and three uncharacterized phospholipids. The genomic DNA G+C content of strain B3T was 42.3 mol%. The DNA–DNA relatedness value between B3T and T. pusilla DSM 9263T was 23.9 %. On the basis of the phenotypic, chemotaxonomic and genotypic characteristics of strain B3T, it represents a novel species of the genus Terasakiella, for which the name Terasakiella brassicae sp. nov. is proposed. The type strain is B3T ( = KCTC 42652T = CGMCC 1.15254T). Emended descriptions of T. pusilla and the genus Terasakiella are also presented.