Abstract 143: Exosomal miRNA Profiling Following Ischaemic Stroke: Clinical and Pre-clinical Data

Introduction: MicroRNA (miRNA) profiles are altered in patients with stroke. Recently, active inter-cellular transport of miRNAs in extracellular vesicles (EV), such as exosomes has been shown. We assessed whether miRNAs packaged in EVs differed in patients with stroke and patients without stroke and then performed in vitro and in vivo experiments to assess potential significance of dysregulated miRNAs. Methods: We recruited patients with suspected stroke. Serum was obtained at 48h post-stroke. EVs were isolated from 200μL serum before RNA extraction. A miRNA microarray was performed (Openarray™ platform) (39 patients) to identify significantly dysregulated miRNAs in stroke vs non-stroke patients. Results were validated by Taqman™ real time quantitative polymerase chain reaction of specific miRNA (17 miRNAs, 169 patients). In vitro studies of selected miRNAs were performed on a rat neuronal cell line (B50) and a rat glial cell line (B92) subjected to 9h hypoxia (1% O2) and serum starvation with 24h reoxyg...