Study on enzymolysis of collagen from sea cucumber intestine and its antioxidant activity.

Objective To investigate the effects of the amounts of papain, pH, hydrolysis time and temperature on the enzymolysis of collagen from sea cucumber intestine. Methods Four kinds of enzyme were discussed on hydrolysis of collagen from sea cucumber intestine, to determine the best one. Hydrolysis of collagen from sea cucumber intestine was performed by papain, and the antioxidant abilities of the hydrolyzate were analyzed by measuring the reducing power on Fe3+, scavenging ability of hydroxyl radicals and DPPH.Results The optimum condition of hydrolysis was 1.0% papain, pH 7.0, with hydrolysis for 4.0h and 65 ℃.Hydrolysis degree was 42.20%. Conclusion The hydrolysis has a good reducing effect on Fe3+and scavenging effect on both hydroxyl radicals and DPPH·.