Overview of the state-of-the-art liposomal drug analysis using high-performance liquid chromatography

ABSTRACTDrug efficacy is improved by selective delivery of the drug to the target by encapsulation within liposomes. Although many new and generic liposomal drugs are under development, only a limited number have been approved. One of the reasons for the small number of successes is the difficulty in analyzing liposomal drugs. In the case of liposomal drugs, the evaluation of various parameters, such as composition, drug amount, size, and surface state, is required for quality control and safety considerations. The fact that these parameters change constantly depending on time and the surrounding environment makes these analyses difficult. This mini review focuses on analytical methods for liposomal drugs that use high-performance liquid chromatography, which is the most popular analytic technique for drug development and quality assurance.