Influence of adsorption-desorption kinetics on the performance of chromatographic processes using large-pore supports

Abstract Large-pore packings are being widely used for bioseparations, e.g. high-pressure liquid chromatography and also in some gas-phase applications. Most studies are based on the assumption of instantaneous equilibrium at the pore-solid interface. This paper shows how adsorption-desorption kinetics influences the performance of chromatographic processes measured by the height equivalent to a theoretical plate (HETP). A criterion is derived allowing us to predict when the assumption of instantaneous equilibrium at the pore-solid interface is valid.