Detection test paper of HER2 protein expression level

The invention provides a detection test paper of HER2 protein expression level, which comprises a base plate, and also comprises an NC membrane, a crystal-marking pad, a water-absorption pad, a sample pad, and a filter membrane. A sample is added and flow laterally to the crystal-marking pad to form two antigen/antibody-crystal compounds; then the two antigen/antibody-crystal compounds continue to flow along the NC membrane in the direction of the water-absorption pad, and reach a detection area; the two antigen/antibody-crystal compounds combines with another two antibodies fixed at the detection area; under illumination, proteins to be detected with different concentrations in the sample are mixed with cellular housekeeper protein-combined crystals, so as to exhibit different colors. The colors are compared with a special colourimetric card by observing the colors of the detection area with naked eyes, and semi-quantitative detection of the breast cancer cell HER2 protein expression is carried out rapidly.