Cloning and sequencing of Gallus chicken MHC-I heavy chain gene.

One pair of primers was designed according to chicken MHC-Ⅰ heavy chain(BF2) gene sequences published in GenBank.Chicken BF2 gene was amplified by RT-PCR from the total RNA of peripheral blood mononuclear cells(PBMCs) isolated from Gallus SPF chicken,and cloned into pGEM-T easy vector.The result showed that the full length of chicken BF2 gene is consisted of 1035 bp,which includes an open reading frame(1035 bp),encoding 345 amino acid residues.The nucleotide sequence of homology to the chicken MHC-Ⅰ heavy chain gene was between 93.0% and 97.6%.The deduced amino acids sequence of homologues to those of the chicken MHC-Ⅰ heavy chain gene were between 83.2% and 97.1%.Comparison of the nucleotides sequence of chicken MHC-Ⅰ heavy chain with those from human and other animals showed that the nucleotides homologues were between 12.9% and 83.6%.