[Influence factors on the cryosurvival rate of post-thaw spermatozoa from sperm donors].

Objective: To investigate the influence of seasons,blood types and semen parameters on the cryosurvival rate of frozen-thawed spermatozoa from sperm donors. Methods: We retrospectively analyzed the influence of seasons,blood types,abstinence period,semen volume,sperm morphology,and pre-freeze sperm motility and concentration on the cryosurvival rate of frozen-thawed sperm in 4 088 semen samples from Shaanxi Human Sperm Bank. Results: The cryosurvival rate of the post-thaw sperm was correlated negatively with the progressive motility of the pre-freeze sperm( r =- 0. 262,P 0. 01),but positively with pre-freeze sperm concentration( r = 0. 247,P 0. 01),and it was significantly higher in the 6-day abstinence group( [70. 2 ± 5. 4]%) than in the other abstinence groups( P 0. 01),so was it in the normal sperm morphology rate 20% group( [71. 4 ± 5. 1]%) than in the others( P 0. 01). The survival rate of the post-thaw sperm was not correlated with seasons,blood types,and semen volume( P 0. 05). Conclusion: Sperm motility,concentration and morphology and the abstinence period of sperm donors may be valuable predictors of the cryosurvival rate of post-thaw sperm,but no correlation was found between the survival rate and seasons,blood types and semen volume.