Biodegradable nano-porous Mn3O4 with sustainable release for improving the stability and bioactivity of peptide RVPSL

Abstract: Food-derived bioactive peptides have drawn much attention because of their health-promoting benefits. The previous study has demonstrated that bioactivity peptide RVPSL could be considered a possible food ingredient. However due to low stability and bioavailability in the gastrointestinal tract limit the application in the food industry. Thus, a biodegradable nano-porous Mn3O4 based on the two-dimensional sacrificial template graphene oxide was synthesized to improve the stability of RVPSL in the gastrointestinal tract. The size of the porous Mn3O4 was approximately 50–150 nm with 5–15 nm pores, and exhibited a high surface area (38.62 m2/g) result to high loading capacity of RVPSL (0.43 mg/1 mg). More importantly, the stability of RVPSL was improved after loaded to the porous Mn3O4. The cumulative release of the functional composite Mn3O4/RVPSL was 31.16% after 120 h incubated in pH 7.4. Furthermore, the bioactivity of peptide RVPSL was improved about 32% after 7 h simulated digestion. In conclusion, the current study demonstrated that the porous Mn3O4 nanoparticle could be considered as a potential strategy for the delivery of bioactive compounds with improved functional attributes.