Fabrication of the graphene honeycomb structure as a scaffold for the study of cell growth

A bio-inspired graphene honeycomb structure was fabricated via the breath figure method using a graphene composite (GO/DODA). The graphene composite was prepared via the phase-transfer method using dimethyldioctadecylammonium (DODA) as the surfactant. The biomimetic structure of the graphene porous film was characterized using optical microscopy, scanning electron microscopy (SEM) and Raman spectroscopy. After reducing the GO/DODA porous film to a rGO/DODA porous film, the cell growth on the honeycomb structure was investigated using two cell lines, HeLa cell and human breast cancer cell (MCF-7) lines. In vitro cell experiments showed that the bio-inspired graphene porous film showed no obvious toxicity to HeLa cells or MCF-7 cells and was promising for biomedical applications.