Determinaton of three kinds of organic acids in the Leaves of Elaeagnus angustifolia L. from different regions and different growth stages in Xinjiang Province by using high-performance thin-layer chromatography

High-performance thin-layer chromatography, which is a quick qualitative and quantitative method, was used to determine 3 kinds of organic acids, namely, ellagic acid, gallic acid, and ferulic acid, in Elaeagnus angustifolia L. leaves originating from 10 regions in Xinjiang Uygur Autonomous Region and 7 different growth stages in Urumqi, China. The developing solvent was toluene‒ethyl formate‒formic acid (5:6.55:1.49), the temperature was 25 ± 1°C, the humidity was 10%, and the detection wavelength was 290 nm. The linear ranges of ellagic acid, gallic acid, and ferulic acid were 0.0564–0.6768, 0.1006–1.2072, and 0.1004–1.2048 μg, respectively, and the linear correlation coefficients were 0.9953, 0.9993, and 0.9970, respectively. The results showed good linear relationships. Precision analyses revealed a relative standard deviation (RSD) of <2.00%. The sample was stable within 24 h at room temperature at RSD ≤3.00%. The average recoveries of ellagic acid, gallic acid, and ferulic acid were 101.4%, 100.9%, ...