[The determination of trypsin activity in duodenal fluid with N alpha-(p-toluenesulfonyl)-L-arginine-p-nitranilide (L-TAPA)].

: The conditions are optimized for the determination of trypsin activity in duodenal juice at 37 degrees C using L-TAPA as a substrate ("Bio-La-Test Trypsin", Lachema, CSSR). Most of the directions of the kit could be accepted, but optimal pH was 0.4 units below that described there. This method allows a highly specific, sensitive, and sufficiently precise determination of trypsin activity in duodenal juice.