Razvoj elektroanalitičke metode cikličke voltametrije za karakterizaciju kafeina u uzorcima dodatka prehrani

Cyclic voltammetry is an electroanalytical procedure in which the measurement is performed in an electrochemical cell. The electrochemical cell consists of a working, auxiliary and reference electrode connected to a voltammetry device. The excitation signal by this procedure is a linearly increasing potential, and the response signal is a current. The potential of the working electrode changes during polarization at a certain speed and in a certain range of potential, where a redox reaction takes place on the surface of the working electrode. This method is used to investigate the mechanisms of reversible, irreversible and quasi – reversible electrochemical processes and for qualitative and quantitative analysis in different systems. Therefore, the task of this thesis is to develop an electroanalytical procedure of cyclic voltammetry, examine the electrochemical behaviour of caffeine solution at different pH values of inert buffer, change of concentration of caffeine solution, change of the polarization rate, and the effect of multiple consecutive polarization and apply to identification and quantification in a real system such as a dietary supplement.