Learning deficits in first generation OF1 mice deficient in (n-3) polyunsaturated fatty acids do not result from visual alteration

The effects of (n-3) polyunsaturated fatty acids (PUFA) diet deficiency on learning, electroretinogram and retinal fatty acid composition were assessed for the first time in OF1 mice. Pups fed the same diets (deficient in α-linolenic acid or a control) as their dams were used aged 7 weeks for passive avoidance test and fatty acid analysis of retinal phospholipids. Visual function was measured by electroretinography in 4- and 7-week-old mice. The (n-3) PUFA-deficient diet significantly decreased learning performance and retinal docosahexaenoic acid level in adult mice. The electroretinogram showed a significant alteration of b-wave amplitude in deficient mice at 4 weeks but not at 7 weeks. These results show that learning deficits in mice fed a diet deficient in (n-3) PUFA were not due to visual alteration.