Method for simultaneously determining four optical isomers of palonosetron hydrochloride

The invention discloses a method for simultaneously determining four optical isomers of palonosetron hydrochloride. The four optical isomers are S.S, S.R, R.S, R.R respectively; and the method is characterized by comprising the following step of splitting the optical isomers of the palonosetron hydrochloride by using efficient liquid chromatography, wherein an OD-H chiral chromatographic column is included, and a mobile phase consists of acetonitrile, isopropanol and diethylamine in a volume ratio of (82-92): (18-8): (0.1-0.2), and the column temperature is 30 to 40 DEG C. A flow rate in the efficient liquid chromatography is 0.3 to 1.2 ml/min. The detection wavelength in the efficient liquid chromatography is 220nm. The method is simple, convenient and practical. The purpose of simultaneously determining and separating the four optical isomers can be rapidly achieved, a basis is provided for quality control of palonosetron hydrochloride raw materials and preparations, and the quality control of a new medicine is facilitated.