Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması

Watermelon seedling blight and fruit blotch caused by Acidovorax citrulli ( Ac ) is a seed-borne disease. The bacterium survives in/on seeds for years. The aim of t his study was to determinate the most favorable, reliable and fast method for detection Ac from cucurbit seeds. Cucurbit seeds were digested using a blender and shaked for 24 h in a nutrienth broth. The methods seedling gow out, pathogen isolation from seed dilutions onto semi selective media P-278 and mEBB and general medium King B, DAS-ELISA and BIO-PCR were used for detection. Water-soaked lesions observed on cotyledons. Since DAS-ELISA and PCR tests with seed dilutions onto King B has failed to detect Ac from seedlots, Ac spesific PCR with dilutions from semi selective media has successfully detect the pathogen. As a result of this study, four (one cucumber, one melon and two watermelon seedlots) out of twenty two tested cucurbit seeds were contaminated with Ac . This study recommended that this BIO-PCR is safe, reliable and fast for detection Ac from cucurbit seeds without any seedling growth.