Binding of calcium, magnesium, and target peptides to Cdc31, the centrin of yeast Saccharomyces cerevisiae.

9 ABSTRACT: Cdc31, the Saccharomyces cerevisiae centrin, is an 10 EF-hand calcium-binding protein essential for the cell division 11 and mRNA nuclear export. We used biophysical techniques to 12 investigate its calcium, magnesium, and protein target binding 13 properties as well as their conformations in solution. We show 14 here that Cdc31 displays one Ca 2+ /Mg 2+ mixed site in the 15 N-terminal domain and two low-affinity Ca