Method for assist-breeding low erucic acid, low sulfuric glucoside cabbage type rape self-incompatible line with microspore cultivation and SSR making

The invention belongs to the technology field of the cole breeding, in particular to the method of the seed selection of the Brassica napus self incompatible line with low erucidic acid and sulfuric glucoside utilizing microspore culture and SSR marker assistance. The method is characterized in selecting the Brassica napus self incompatible line S-1300 as the female parent and maintenance line 04P63 External-52 as the male parent, crossbreeding and obtaining F1, microspore culture F1 and obtaining double haploid (DH) self incompatible line; further screening and obtaining the DH material of the Brassica napus self incompatible line with low erucidic acid and sulfuric glucoside; analyzing the variation of the self incompatible double haploid (DH) line by using the SSR markers, studying six major traits of the self incompatible double haploid (DH) line including the plant height, the first branch number, the first branch silique number, main inflorescence length, main inflorescence silique number and yield per plant; screening the double-low Brassica napus self incompatible double haploid (DH) line superior to the S-1300 in production, related trait and general combining ability. The seed selection provides an easy, rapid and effective method of the double-low Brassica napus inbred incompatible double haploid (DH) line.