High Pressure Homogenization Extraction and Purification of the Main Functional Components from Medlar Leaves of Qaidam Basin

This paper,examines the optimum process involved in the high pressure homogenized extraction of the main functional components from medlar leaves of Qaidam Basin,during the process of which the extract was purified under the optimum condition.The extraction condition was optimized by orthogonal experiment on four single factors including ethanol concentration,solid-liquid ratio,extraction pressure,and extraction time.The optimum condition was as follows:ethanol volume fraction:80%,solid-liquid ratio:1∶10,extraction pressure:60 MPa,extraction time:30 min.Under such optimum condition,the rutin mass fraction of the extract was 10.53% and the total flavonoids mass fraction was 32.61%.The condition of purification process was:AB-8 macroporous resin was selected for use,and some parameters were determined in purification technology:the volume of water:5 BV,the concentration of used ethanol gradient and the adding volume:4 BV.Under such conditions,rutin mass fraction of the purified product was up to 60.85% and the total flavonoids mass fraction was up to 90.53%.