Study on Derivatising Condition of Cadaverine with Dansyl Chloride as Pre-Column Derivatising Agent

A rapid ultrasonic wave-assisted dansylation procedure was developed for the derivatization of cadaverine prior to HPLC determination. Cadaverine was quantitatively dansylated in 10 min using a ultrasonic power of 200 W and a operating frequency of 40 KHz .In this case, the excellent derivatising condition are determined as follows: pH of buffer, 9.5; concentration of dansyl chloride, 5 mg/mL; time of reaction, 10 min. This method is fast, reproducible and practical for the analysis of cadaverine.