Evaluation of biochemical alterations produced by combined exposure of fenvalerate and nitrate in Bubalus bubalis.

Aim: Evaluation of combined effect of fenvalerate and nitrate on biochemical parameters in buffalo calves. Materials and Methods: Sixteen male buffalo calves were divided into four groups of four calves each. Group I receiving no treatment served as the control. Group II and III animals were orally administered with fenvalerate (1.0 mg/kg/day) and sodium nitrate (20 mg/kg/day), respectively, for 21 consecutive days and were kept as positive control. Group IV animals were co-administered with fenvalerate and sodium nitrate at the above dose rates for 21 consecutive days. Biochemical parameters including Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Gamma-glutamyl transpeptidase (GGT), Lactate dehydrogenase (LDH), Glucose, Total protein, Albumin, Cholesterol, Blood urea nitrogen (BUN) and Creatinine were th determined on 0, 3, 7, 10, 14, 17 and 21 day of treatment. Estimation of these parameters was also done on 7 day of posttreatment period. Results: Co-administration of fenvalerate and sodium nitrate produced significant increase in the plasma levels of AST, ALP, GGT, LDH, glucose, BUN, cholesterol and creatinine while significant decrease in the plasma levels of total proteins was observed. No significant alteration was observed in albumin levels. Extent of organ damage as evidenced by biochemical alterations was more pronounced in calves exposed to combination of fenvalerate and sodium nitrate as compared to their individual exposures. Conclusion: Fenvalerate and sodium nitrate co-administration potentiates the toxicological injury produced, in comparison to their individual exposure.