Influence of Stent Edge Angle on Endothelialization in An In Vitro Model

PURPOSE To investigate the influence of topographic features in the path of migrating endothelial cells, specifically the effect of edge angle of intravascular metallic material on endothelialization. MATERIALS AND METHODS Flat 1-cm × 1-cm 316-L pieces of stainless steel were placed on confluent monolayers of human aortic endothelial cells. The thickness of each metal piece was ground to achieve an edge angle of 35°, 70°, 90°, or 140° ( n = 6 each) in relation to the endothelial surface. Migration distance and density of endothelial cell coverage on the metal pieces were measured in groups of six each under static conditions at 4, 7, and 11 days and flow conditions (16 dynes/cm 2 ) at 4 days. RESULTS Endothelial cell migration distance along the surface of the pieces with edge angles of 35° was significantly greater than that with those with larger angles ( P CONCLUSIONS The edge angle of intravascular metallic material has an influence on the rate of endothelialization. A smaller edge angle facilitates endothelialization over metallic material when compared to a larger angle. These results demonstrate the importance of metallic stent profile on endothelialization rate.