[Effect of the C-terminal truncated human apoptosis-inducing factor δ1-480 on biological behaviors of MCF-7 cells].

Objective To observe the expression of the C-terminal truncated human apoptosis-inducing factor(AIF) and its biological effect on MCF-7 cells.Methods PcDNA3.0-FDT-AIFΔ1-480 was transfected into human breast carcinoma MCF-7 cells with lipofectamins.The expression of the truncated AIF gene was detected by Western blotting,and its effects on the biological behaviors of MCF-7 cells and on the expression of cytochrome c(cytC) were evaluated using flow cytometry,MTT assay,colony-forming assay,and mitochondrial membrane potential measurement.Results PcDNA3.0-FDT-AIFΔ1-480 enhanced AIF expression in MCF-7 cells,obviously inhibited the cell proliferation,and significantly reduced the mitochondrial membrane potentials(P0.05).Transfection of the cells with PcDNA3.0-FDT-AIFΔ1-480 promoted the expression of cytC and resulted in significantly increased apoptosis of MCF-7 cells(P0.05).Conclusion The expression of C-terminal truncated human AIF gene can induce apoptosis of human MCF-7 cells by promoting cytC release from mitochondria.