Effects of microRNA-494 on apoptosis and invasion against osteosarcoma cell through regulation of Survivin

Objective To investigate the effects of microRNA (miRNA, miR)-494 on the proliferation, apoptosis and invasion against osteosarcoma MG-63 cell through regulation of Survivin. Methods The expression of miR-494 in osteosarcoma MG-63 cells was detected by real-time quantitative polymerase chain reaction (Real-time PCR) after miR-494 mimic transfection. After the validation of miR-494 in this model, cell count kit-8 (CCK-8) and flow cytometry assay were used to detect the proliferation and apoptosis after miR-494 mimic transfection; Transwell assay was used to determine the invasion MG-63 cells. Then the expression levels of Survivin mRNA and proteins were detected by Real-time PCR and Western blotting respectively. Results After miR-494 mimic transfection in MG-63 cells, relative expression of miR-494 in mimic group (4.38±0.53) significantly increased compared with the negative-control (NC, 1.02±0.02) group (t=6.312, P=0.003). The proliferation 24 h and 48 h after miR-494 mimic transfection were significantly suppressed compared with the NC group. The flow cytometry assay suggested the apoptosis cell increased in mimic group (8.76±0.82)% compared with the NC group [(5.43±0.63)%, t=3.199, P=0.032]. Transwell assay showed the invated cells significantly reduced in mimic group (60.40±11.28) compared with the NC group (109.51±7.46, t=3.628, P=0.022). The expressions of Survivin in the mRNA and protein levels were reduced significantly in mimic group after transfection. Conclusion miR-494 inhibited the proliferation, invasion cell and induced apoptosis of osteosarcoma MG-63 through the down-regulation of Survivin. Key words: Osteosarcoma; MicroRNA-494; Survivin; Proliferation; Apoptosis; Invasion