Působení desinfekčních prostředků a detergentů na přežívání arkobakterů ve vodách

The subject of this thesis was to study the effects of disinfectants and detergents on the survival of arcobacters in different water environment. There was used cultivation method and PCR method during the testing of cell survival of arcobacters. The operating concentrations of disinfectant used in food plants were tested, Chloramine (1 %), Oxonia (1 %), Topax (3 %) and Torsan (3%). Reference disinfections have been effective for both bacterial strains Arcobacter butzleri CCUG 30484 and Arcobacter cryaerophilus CCM 3934. Further, the minimum inhibitory concentration of the disinfectant and detergents was determined. Minimum inhibitory concentration of both strains was 0.2 % for Chloramine and Oxonia less than 0.1 % after 30 minutes, Topax and Torsan disinfections had different minimum inhibitory concentrations for the reference strains, 1.25 % concentration of Topax and 1 % concentration of Torsanu for Arcobacter butzleri CCUG 30484, for Arcobacter cryaerophilus CCM 3934 it was 0.5 % concentration of Topax disinfectant and 0.8% of Torsan disinfectant. For surfactants, the minimum inhibitory concentration after 30 minutes for Palmex was 0.2 %, for Vanish less than 0.1 % and for Woolite the value could not be determined because neither a 4% concentration did not inhibic the growth. The sterile and real water samples which were artificially contaminated by bacteria arkobacters were monitored by PCR method for their efficiency of disinfection and surfactants in different concentrations of Chloramine (0.2 and 1 %), Palmexu (0.2 %), Vanish (0.1%), and Woolite (4 %) at temperatures 15 ° C and 30 ° C at different times. After 30 minutes it was found out that cells of Arcobacter cryaerophilus CCM 3934 were inhibited in the two tested concentrations of Chloramine. Cells of Arcobacter butzleri CCUG 30484 were much more resistant. The surfactants, which were observed, show that they are not as effective as chloramines. Repeatedly, it was confirmed that Arcobacter cryaerophilus CCM 3934 is sensitive because in samples with sterile water with Palmexu (0.2%), and Vanish (1%)was not identified while Arcobacter butzleri CCUG 30484 was detected at these concentrations.