e0050 Effect of Bosentan on carotid artery restenosis in rat and correlation of serum VEGF

Objective To investigate the effect of Bosentan on carotid artery restenosis in rat and correlation of serum vascular endothelia growth factor (VEGF). Methods 36 Wistar rats were divided randomly into three groups: control group (n=12), angioplasty group (n=12) and Bosentan (BA) with angioplasty group (n=12). Bosentan was administrated del to rats of BA with angioplasty group. The balloon catheter injury was performed on left common carotid artery of rat by imitating the process of angioplasty. After 7 days and 14 days, neointimal area (NIA) and stenosis index (SI) were calculated. Prior to angioplasty and 7 days after balloon injury, serum VEGF were assessed. In another experiment, 65 Wistar rats were divided randomly into three groups: control group, angioplasty group and Bosentan with angioplasty group. Bosentan was administrated to rats of Bosentan (BA) with angioplasty group. The balloon catheter injury was performed on left common carotid artery of rat by imitating the process of angioplasty. The process of neointimal and media hyperplasia was observed and α-action and PCNA expressions were determined. Results NIA and SI of BA with angioplasty were significantly decreased compared with angioplasty group (p Conclusions Bosentan may be effective on artery restenosis by inhibiting neointimal hyperplasia, increasing the serum VEGF level, reducing the proliferation, migration and transconformation of vascular smooth muscle cells.