Associations of Gene Polymorphism in Kazakhstani Patients with Implanted LVAD

Purpose Heart failure is one of the main problem in the health care system. The best option of the treatment is heart transplantation (HT). Left ventricular assist device (LVAD) implantation is the best option of alternative treatment instead of HT. Outcome of the LVAD treatment is usually followed with side effects of bleeding and thrombosis. To reduce side effects patients are prescribed with antithrombotic therapy warfarin, which can be followed with side effects because of incorrect individual dosage. Warfarin dosage can be identified according to the results of genetic polymorphisms CYP2C9 and VKORC1. The study is aimed to identify genetic associations of SNP polymorphisms rs1799853, 1057910, rs9923231. Methods Venous blood samples were recruited from 98 patients with implanted LVAD at the National Research Center for Cardiac Surgery. Heart failure patients contained 92 males and 6 females (52.7±10.95 years old) with implanted LVAD devices. The control group contains ninety-five (n=95) conditionally healthy individuals. Warfarin treatment was prescribed after LVAD implantation monthly (after 1st month, 3rd, 6th, 9th and after 1 year etc). SNP polymorphisms were genotyped in case and control groups by real-time polymerase chain reaction with TaqMan probes. Results Genotyping test results were not statistically significant. Case and control groups did not have mutant type of genotypes TT (rs1799853) and CC (rs1057910). Genotype frequency of CC genotype rs1799853 was slightly higher in the case group (92.9% vs.92.6%, p = 1.000). The second SNP rs1057910 had higher frequency of AA genotypes in case group (92.9% vs. 86.3%, p = 0.161). Mutant genotype TT of rs9923231 was slightly higher in the control group (37.9% vs.36.7%, p = 0.718). Monthly clinical dosage of warfarin was compared between three genotypes of rs9923231. Warfarin dosage was significantly associated between three genotypes (p Conclusion Research need to include other polymorphisms for deeper research about individual warfarin dosage and their associations with side effects.