Serodiagnosis of Przhevalskiana spp infestation in goat using a Competitive ELISA

ABSTRACT: Goat warble-fly infestation is one of parasitic disease that larvae of przhevalskiana spp are known as factor for its causes. It is known to be a major challenge for health and welfare in infested goats and causes high economic losses in livestock all over world. Detection of goat grub was previously done by direct palpation of second and third stage larvae in the back and flank site of the live animals or visual evaluation of the carcases in slaughterhouses but due to small size of the first instar larvae of przhevalskiana (less than 1 mm during emerging from the egg), some of the infected cases were ignored and recorded as negative samples. Immunodiagnosis procedure for myiasis help to detect the easy and economical in living animals (even when larvae are still migrating or otherwise undetectable in the animal body). This study was conducted to evaluate the competitive ELISA system in order to detect the antibodies of przhevalskiana larvae in goat sera. In order to prepare the larval antigen 200 first instar larvae of przhevalskiana were collected from the subdermal region of the back and flanks of infested goats from Khoramabad slaughter house during September 2017 to March 2018. And totally, 37 sample sera were tested from infested goats along with 64 sera from uninfected goat. The sensitivity and specificity at cut-off 3SD were 89.18% and 84.78%. Also, the measures of inter-and intra assay coefficients of variability to express the precision or replicability of ELISA kit results were 5.33% and 2.81%, respectively.