Comparison of Two Maize SSR Detection Methods: Capillary Electrophoresis with Fluorescence Detection Method and Denaturing PAGE Silver-staining Detection Method

Seven SSR primers and 192 maize hybrid were used to comparatively analyze SSR markers between capillary electrophoresis with fluorescence detection and denaturing PAGE(polyacrylamide gel electrophoresis) silver-staining detection.The results indicated that SSR markers fragment sizes explored by two decetion systems were relatively similar.Capillary electrophoresis with fluorescence detection has the advantages of high accuracy,sensitiveness,cost effectiveness and high-throught.Therefore,the capillary electrophoresis with fluorescence detection system is suggested to be suitable for scaning and analyzing large-scale material.While denaturing PAGE silver-staining detection would be used in examaining a few samples,especially in screening SSR markers.