Sterol transfer by atypical cholesterol-binding NPC2 proteins in coral-algal symbiosis

Coral reefs are the most biodiverse marine ecosystems on our planet. Their immense productivity is driven by friendly relationships, or symbioses, between microbes called algae and the corals. Related organisms, such as anemones, also rely on these close associations. The algae use energy from sunlight to make sugars, cholesterol and other molecules that they supply to their host. In exchange, the host’s cells provide homes for the algae inside specialist, acidic structures called symbiosomes. Corals and anemones particularly need cholesterol and other ‘sterol’ molecules from the algae, because they are unable to create these building blocks themselves. In mammals, a protein known as Niemann-Pick Type C2 (NPC2) transports cholesterol out of storage structures into the main body of the cell. Corals and anemones have many different, ‘atypical’ NPC2 proteins: some are produced more during symbiosis, and these are mainly found in symbiosomes. However, it was not known what role these NPC2 proteins play during symbioses. Here, Hambleton et al. studied the symbioses that the anemone Aiptasia and the coral Acropora create with different strains of Symbiodiniaceae algae. The experiments found that the strain of algae dictated the mixture of sterols inside their hosts. The hosts could flexibly use different mixes of sterols and even replace cholesterol with other types of sterols produced by the algae. Atypical NPC2 proteins accumulated over time within the symbiosome and directly bound to cholesterol and various sterols the way other NPC2 proteins normally do. Further experiments suggest that, compared to other NPC2s, atypical NPC2 proteins may be better adapted to the acidic conditions in the symbiosome. Taken together, Hambleton et al. propose that atypical NPC2 proteins may play an important role in allowing corals to thrive in environments poor in nutrients. The first coral reefs emerged over 200 million years ago, when the Earth still only had one continent. Having built-in algae that provide the organisms with nutrients is thought to be the main driver for the formation of coral reefs and the explosion of diversity in coral species. Yet these ancient relationships are now under threat all around the world: environmental stress is causing the algae to be expelled from the corals, leading to the reefs ‘bleaching’ and starving. The more is known about the details of the symbiosis, the more we can understand how corals have evolved, and how we could help them survive the crisis that they are currently facing.