GENETIC POLYMORPHISM OF SOME MICROSATELLITES ON CHROMOSOME SEVEN IN THE EGYPTIAN BUFFALO

Five bovine microsatellites were tested in the Egyptian buffalo genome to determine the amplification as well as the number of alleles if amplification was successful. These microsatellites, BM143, TGLA37, FBN13, ILSTS035 and ILSTS093 were tested in 70 lactating Egyptian buffaloes. All microsatellites tested amplified the buffalo DNA and produced a PCR product. Characterization of the PCR products on polyacrylamide gels revealed that three of the microsatellites tested were monomorphic (TGLA37, FBN13, ILSTS035), while the other two were polymorphic (BM143, ILSTS093). BM143 microsatellite had nine alleles with size ranging from 99 and 135 bp. Allele frequency ranged from 0.015 to 0.431. ILSTS093 showed eight alleles ranging in size from 193 to 217 bp with allele frequency ranging between 0.009 and 0.293. The results obtained indicated the successful genotyping of bovine microsatellites in the buffalo genome and suggested that some cattle microsatellites can be a valuable source for genome analysis in the Egyptian buffalo.