Effects of non-coding RNA00460 targeting microRNA-539/SRY-related high mobility group-box gene 4 axis on proliferation and migration of glioma cells

Objective To investigate the effects of non-coding RNA00460 targeting microRNA (miRNA, miR)-539-SRY-related high mobility group-box gene 4 (SOX4) on the proliferation and migration of glioma cells. Methods Twenty cases of normal brain glia and 20 cases of glioma were collected. The expression levels of lncRNA 00460 and miR-539 were analyzed by fluorescence quantitative polymerase chain reaction (PCR). Bioinformatics was used to analyze the relationship between lncRNA 00460 and miR-539 and target genes; knockdown cell line lncRNA 00460 and miR-539 overexpression cell line were constructed in U251 glioma cell line. cell counting kit-8 (CCK-8) and Transwell were used to analyze the proliferation and migration ability of glioma cells treated with different methods; xenograft tumor model was used to analyze the proliferation ability of glioma cells in vivo. Results Compared with normal brain glioma cells [(1.21±0.25), (1.35±0.29)], the expression of lncRNA 00460 (3.41±0.51) was significantly increased in glioma tissues, while the expression of microR-539 (0.39±0.11) was significantly decreased (t=3.189, 4.208, P<0.05). Bioinformatics studies have found that lncRNA 00460 regulates the expression of miR-539 and further regulates the expression of SOX4 protein. The knockdown of lncRNA 00460 significantly inhibited the proliferation and migration of glioma cells (F=3.729, 3.109, P<0.05). Overexpression of miR-539 significantly increased the proliferation and migration of glioma cells (F=5.129, 5.114, P<0.05). In vivo xenogeneic tumorigenesis model showed that knockdown of lncRNA 00460 significantly inhibited the growth of glioma cells in vivo, while over-expression of microRNA539 significantly promoted the proliferation of glioma cells (F=5.194, 3.104, P<0.05). Conclusion lncRNA 00460 regulates the proliferation and migration of glioma cells by targeting the miR-539/SOX4 axis. Key words: Long non-coding RNA 00460; MicroRNA-539; Glioma cells; Proliferation; Migration