Development of Preparation Technology,Safety and Potency Tests of the Vaccine of Propolis Adjuvant Inactivated Vaccine against Newcastle Disease in Poultry

batches of the propolis Adjuvant inactivated vaccine against Nescastle disease were producted with Nescastle dissease virus Clone_30.The former was harvested from the infected experimentally chicken embryos.No abnormal signs were observed on the 10 aged 3～6 weeks old chickens injected with 1ml of the vaccine (i.e.2 immune dose)and no influences on the body weight gain curve in the following 1～15 days after injected with 0.5ml Vaccine (i.e.one immune dose)at the one day old were manifested by the 10 chicks.Vaccination of the 3～6 weeks old chickens with the vaccine induced the hemagglutination inhibition(HI)antibody titer to ND in geometric mean 4.8～9.8log2 in the following 5～32 days by the 3 batches respectively.The 50%protective dose per vaccine dose(PD 50 /dose)were≥127and≥131 respectively.