Purification and identification of Yersinia outter-membrane protein 1

The gain of pure protein 1 from 1 litre culture was 6.3mg. It would show 3 visual bends on SDS-PAGE gel and their molecular weight were 150, 90-100 and 45-50 KD respectively. It was decuced that the subunit molecular weighgt of this protein was 45-50KD and the 150 and 90-100 KD bends were represent their duplet and triplet forms respectively.