[Sequence detection of HBV-DNA P and C region in HIV/HBV superinfection subjects with drug resistance to HAART].

OBJECTIVE: To further study the resistance of HBV to high activity antiretrovirus therapy(HAART). METHODS: HBV-DNA was quantitatively detected by real-time PCR in 36 HIV/HBV superinfection subjects, C region and P region HBV-DNA in high copies HBV-DNA subjects were detected by routine PCR, PCR products were purified and sequenced and compared with the HBV international Genbank using BLSAT softy ware. RESULT: HBV-DNA was positive in 4 of 36 patients (11.1%) and another 3 had low copies(<10(4)copies/ml), one had a high HBV-DNA copies (10(7)copies/ml). It's HBV-DNA C region sequence had mutation on 2 sites (nt 2412 T/C; nt 2413 T/C) and 1 mutation P region (nt 741 A/G, also YMDD/YVDD) compared with HBV international Genbak reference sequence. CONCLUSION: The HBV resistance to HAART may be related with multiple genetic mutations in the C and P regain of HBV-DNA.