Transferability of heterologous microsatellite primers in Brycon gouldingi

EnglishBrycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were assessed. Among the 30 primers, seven showed satisfactory transferability, six of which belonged to the genus Brycon: Bh13 (B. hilarii), BoM5, BoM13 (B. opalinus), Borg9, Borg13, and Borg59 (B. orbignyanus), and one belonged to P. argenteus (Par80). The primers for the other species tested showed non-specificity or monomorphism; and were therefore, excluded from the analyses. The number of alleles ranged between two (Borg13 and Borg59) and three (Bh13, BoM5, BoM13, Borg9 and Par80), with sizes varying between 103 bp (BoM5) and 430 bp (Borg9). Four primers showed evidence of null alleles (BoM13, Borg9, Borg13, and Par80), which could probably be attributed to the respective Hardy-Weinberg deviation. Thus, seven primers were validated for cross-amplification in B. gouldingi, which may be used in future studies involving this species. Key words: conservation; cross-amplification; molecular markers; SSR portuguesBrycon gouldingi e uma especie de peixe neotropical com importância socioeconomica e ambiental na Bacia do Tocantins-Araguaia. Estudos geneticos nessa especie ainda sao escassos, dificultando o conhecimento sobre a estrutura populacional e a diversidade genetica nos estoques naturais e em cativeiro. O objetivo do presente estudo foi avaliar a transferibilidade de primers microssatelites heterologos em B. gouldingi. Foram avaliados um total de 30 primers de oito especies: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus e Colossoma macropomum. Os primers que demonstraram melhores padroes de amplificacao foram aplicados em 20 especimes de B. gouldingi para os calculos dos parâmetros geneticos. Sete dos 30 primers apresentaram resultados satisfatorios de transferibilidade, sendo seis oriundos do genero Brycon: Bh13 (B. hilarii), BoM5, BoM13 (B. opalinus), Borg9, Borg13 e Borg59 (B. orbignyanus), e um oriundo de P. argenteus (Par80). Os primer das outras especies testados mostraram inespecificidade ou monomorfismo, sendo excluidos das analises. O numero de alelos variou de dois (Borg13 e Borg59) a tres (Bh13, BoM5, BoM13, Borg9 e Par80), com tamanhos entre 103 pb (BoM5) e 430 pb (Borg9). Quatro primers apresentaram evidencias de alelos nulos (BoM13, Borg9, Borg13 e Par80), o que provavelmente inferiu sobre o desvio de Hardy-Weinberg nos mesmos. Concluindo, sete primers foram validados para a amplificacao cruzada em B. gouldingi e poderao ser utilizados em futuros estudos com essa especie. Palavras-chave: amplificacao cruzada; conservacao; Marcadores moleculares; SSR