Effect of soil contamination with azadirachtin on dehydrogenase and catalase activity of soil

Insecticides are used in modern agriculture in large quantities to control pests and increase crop yield. Their use, however, has resulted in the disruption of ecosystems because of the effects on non-target soil microorganisms, some environmental problems, and decreasing soil fertility. These negative effects of synthetic pesticides on the environment have led to the search for alternative means of pest control. One such alternative is use of natural plant products such as azadirachtin that have pesticidal activity. The aim of this experiment was to study the effect of soil contamination by azadirachtin (C 35 H 44 O 16 ) on dehydrogenase (DHA) and catalase activity (CA) of soil under field conditions in Perm, Russia. The tests were conducted on loamy soil (pH H2O 6.7, EC H2O 0.213 dSm -1 , organic carbon 0.99%), to which the following quantities of azadirachtin were added: 0, 15, 30 and 60 mL da -1 of soil. Experimental design was randomized plot design with three replications. The DHA and CA analyses were performed 7, 14 and 21 days after the field experiment was established. The results of field experiment showed that azadirachtin had a positive influence on the DHA and CA at different soil sampling times. The increased doses of azadirachtin applied resulted in the higher level of DHA and CA in soil. The soil DHA and CA showed the highest activity on the 21th day after 60 mL azadirachtin da -1 application doses.