Nematicidal effects of Acacia nilotica, Azadirachta indica, Brassica chinensis and Ecklonia maxima against soybean cysts nematode.

The effect produced by aqueous extract of Acacia nilotica, Azadirachta indica, Brassica chinensis, and Ecklonia maxima in the control of soybean cysts nematode (SCN) was tested in laboratory and green house. SCN eggs, second stage juvenile and soybean plants inoculated with 1000 eggs were treated with extract of each of the plant material at concentrations of 120 mug/mL and 12 mug/mL. Results obtained showed all plant materials significantly reduced H. glycine population compared to control. A. indica at concentration of 120 mug/mL exhibited highest inhibition to egg hatch, juvenile mortality, reduction in number of females and cysts of 75.21%, 100%, 83.12% and 80.17%, respectively. This was closely followed by B. chinensis, A. nilotica and E. maxima. The aim is to study the efficacy of each plant material for the possibility of mixing their crude form to formulate an organic nematicide alternative to methyl-bromide that was complete banned globally in 2005 due to its harmful effect on the ozone layer. A. indica and B. chinensis showed high potential nematicidal properties, which could be explored further in effort to formulate organic nematicide from mixture of two the two plant materials in order to benefit from synergistic and potentiating effect.