To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments. Chemicals/CAS: isobutyric acid, 5711-69-3, 79-31-2; isovaleric acid, 503-74-2, 539-66-2