Characterization and differentiation of normal and abnormal spermatozoa via micro-Raman spectroscopy
2013
Growth in the percentage of spermatozoa with aberrant sperm head morphologies has been correlated with a rise in male infertility. In our study, micro-Raman spectroscopy using a new substrate was utilized to characterize and differentiate the morphologically normal and abnormal human sperm cells based on their different biochemical components by showing their different specific Raman spectra. A detailed classification based on the PCA?LDA method was performed showing a diagnostic sensitivity of 76% and specificity of 91%, with 80% classification accuracy. Our results suggest that micro-Raman spectroscopy can serve as a reliable and non-invasive tool for detection and differentiation of human spermatozoa.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
21
References
9
Citations
NaN
KQI