Erratum to "Genetically encoded probe for fluorescence lifetime imaging of CaMKII activity" [Biochem. Biophys. Res. Commun. 369 (2008) 519-525] (DOI:10.1016/j.bbrc.2008.02.070)

Biochemical and Biophysical Research Communications 373 (2008) 179 Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.elsevier.com/locate/ybbrc Erratum Erratum to ‘‘Genetically encoded probe for fluorescence lifetime imaging of CaMKII activity” [Biochem. Biophys. Res. Commun. 369 (2008) 519–525] Showming Kwok a , Claudia Lee b , Susana A. Sanchez b , Theodore L. Hazlett b , Enrico Gratton b , Yasunori Hayashi a, * a RIKEN-MIT Neuroscience Research Center, The Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, 77 Massachusetts Avenue 46-4243A, Cambridge, MA 02139, USA b Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, 3120 Natural Sciences II, University of California-Irvine, Irvine, CA 92697-2715, USA Due to a printer’s error, Fig. 1A did not reproduce well in the printed issue. For the reader’s convenience, Fig. 1 is provided here. Fluorescence (AU) A Venus/CFP mRFP/GFP 2 - T305D/T306D mRFP/GFP GFP/mRFP Before Ca 2+ + EGTA Wavelength (nm) Ca 2+ EGTA Ca 2+ Ca 2+ EGTA EGTA EGTA n=3 Time (min) D mRFP/GFP n=3 Donor Acceptor n=3 n=6 C Ca 2+ 25 min 10 μm GFP fluorescence (AU) Fluorescence (AU) B 4Br-A23187 n=5 Time (min) Fig. 1. mRFP/GFP-Camui showed a significant dequenching upon stimulation. (A) The emission spectra of the Venus/CFP-Camui, mRFP/GFP-Camui, mRFP/GFP-Camui with T305D/T306D mutations and GFP/mRFP-Camui made with the HEK293T cell lysate expressing each construct before, 3 min after the addition of 1 mM Ca 2+ , and 3 min after the addition of 1.5 mM EGTA, at the donor specific excitation. The expected positions of acceptor peaks are shown by downward arrows. A 10-fold magnification of the mRFP emission peak is shown in the inset for mRFP/GFP-Camui. (B) A plot of donor and acceptor peak intensity over time, normalized by the donor peak intensity before application of Ca 2+ . The response was monitored every 1 min (C). GFP fluorescence image of HEK293T cells expressing mRFP/GFP-Camui. (D) A summary of GFP fluorescence intensity in HEK293T cells. Intensity was normalized to the average intensity prior to stimulation for each cell. The error bars are SEM. DOI of original article: 10.1016/j.bbrc.2008.02.070 * Corresponding author. E-mail address: yhayashi@mit.edu (Y. Hayashi). 0006-291X/$ - see front matter O 2008 Elsevier Inc. All rights reserved. doi:10.1016/j.bbrc.2008.05.109