Association of rs7041 and rs4588 Polymorphisms of the Vitamin D Binding Protein and the rs10741657 Polymorphism of CYP2R1 with Vitamin D Status Among Jordanian Patients.

Aim: Previous studies have shown a high prevalence of vitamin D deficiency among Jordanians despite adequate exposure to sunlight, suggesting the presence of other causes for this deficiency. The aim of this study was to identify the relationship between 25-hydroxyvitamin D [25-(OH) VD] status and the nonsynonymous single-nucleotide polymorphisms (SNPs) (rs7041 and rs4588) of the GC gene, which encodes the vitamin D binding protein, and one SNP (rs10741657) near the CYP2R1 gene. Methods: Blood samples from 381 subjects (74 males and 307 females, 18–60 years of age) were obtained from the “National Center for Diabetes, Endocrinology and Genetics” (Amman, Jordan). The subjects were classified as “apparently healthy” if they did not suffer from chronic diseases and as “unhealthy” if they suffered from certain chronic diseases. Subjects' genotypes for GC; rs7041 and rs4588; CYP2R1; rs10741657 were determined by the polymerase chain reaction–restriction fragment length polymorphism assay method. Results: Appar...