Study on Hemorrhagic Fever with Renal Syndrome Surveillance in Hunan Province,2006

Objective To explore the epidemic foci,type of hemorrhagic fever with renal syndrome(HFRS),population distribution,epidemic trend,host animals and their virus-carrying situation,and to take comprehensive scientific measures to prevent and control HFRS in Hunan province.Methods According to Implementation Scheme for Hemorrhagic Fever with Renal Syndrome Surveillance in 2006 in Hunan province,epidemiological and pathogenic sureillance was conducted in seven aeras.Direct immunofluorescenco assay(DFA)was used to detect the antigen form rat lungs.The total viral RNA was extracted from the lung tissues of the Hantavirus-infected rats and amplified with RT-PCR by using the Hantavirus genotype specific primers.The amplified genes were then sequenced and subjected to genotyping.Results A total of 560 cases were reported in 2006,the annual incidence rate was 0.89/100 000.The total number of captured rodents was 654,and the average rodent density was 3.15%.The virus carrying rate of rodents was 1.31%.Genotype analysis revealed 7 Hantavirus type II(SEOV)positive specimens taken from indoor rodents(3 Rattus norvegicus,1 Mus musculus from Liuyang county,1 Rattus flavipectus,1 Mus musculus and 1 Apodemus agrarius from Shaodong county)and 1 Hantavirus type I(HTNV)positive specimen from a outdoor Apodemus agrarius captured from Shuangfeng county.Conclusion Both Hantavirus type II(SEOV)and the Hantavirus type I(HTNV)existed in Hunan province.SEOV was the main genotype Comprehensive measures should be taken to prevent and control HFRS by extinguishing rats,vaccine inoculation for high-risk people and developing the health education.