Determination of Levels of Cerebrospinal Fluid Transferrin,IgG,CRP and α_1-MG in Evaluation of Blood-brain Barrier Injury

Objective To evaluate the determination of cerebrospinal fluid transferrin,immunoglobulin G(IgG),C-reactive protein(CRP) and α1-MG in evaluation of blood-brain barrier(BBB) injury.Methods Nephelometry was used to determine the concentrations of transferrin,IgG,CRP andα1-MG in cerebrospinal fluid of patients with BBB injury.Results With the aggravation of BBB injury,the concentrations of transferrin and IgG increased,and there was significant difference between groups with different degrees of BBB injuries(P0.01).Cerebrospinal fluid transferrin and IgG were positively correlated with albumin quotient(r=0.853,0.834,respectively,P0.01).The ROC curve AUC of transferrin and IgG was 0.992,0.970,respectively,and in diagnosis of BBB injury the sensitivities of transferrin and IgG were 94.2%,94.2%,respectively,and the specificity 95.5%,91.9%,respectively.The cerebrospinal fluid CRP concentration increased in 6 patients,but α 1-MG concentration was under the detectable limitation(5.29 mg/L) in all patients.Conclusion Determination of cerebrospinal fluid is a good indicator reflecting BBB injury.IgG detection can reflect BBB functional status when no immune globulin was produced in central nervous system.The presence of CRP in cerebrospinal fluid shows injured BBB and increased CRP in peripheral blood.The detection of α 1-MG in cerebrospinal fluid is of no significance in evaluation of BBB injury.