Development of a loop-mediated isothermal amplification method with fimY gene for rapid detection of Salmonella in food

According to the highly conserved fimY gene of Salmonella,two pairs of primers were designed and the method for detecting Salmonella in food was developed with loop-mediated isothermal amplification(LAMP).In results,sixty-eight strains of forty bacteria species were used for specificity test and all the tested Salmonella strains were positive and the other strains were negative in LAMP,indicating that the LAMP method was highly specific for target bacteria with the sensitivity of 5 CFU per test tube for cultivated Salmonella and 9 CFU per test tube for its contaminated food.The LAMP test could be completed in 60min.165 from 802 samples of meat,egg,milk and its products,animal diarrhea materials and artificial contamination samples were positive in LAMP,which was in accordance with the testing result according to GB/T4789.4-2008.The results showed that the LAMP assay was a sensitive,rapid and simple tool for detecting Salmonella.