Synthesis of catalase-inorganic hybrid nanoflowers via sonication for colorimetric detection of hydrogen peroxide

Abstract A catalase-inorganic hybrid nanoflowers (Cat-HNFs)-based colorimetric platform was prepared for visual detection of hydrogen peroxide (H 2 O 2 ). Catalase via coordination was self-assembled and embedded in the copper phosphate crystals by sonication. Under the designed colorimetric system, Cat-HNFs showed linear absorbance responses toward H 2 O 2 in the concentration range of 1 μM to 1 mM, and the detection limit was 1 μM. Compared to free catalase, Cat-HNFs exhibited enhanced activity and stability for H 2 O 2 detection. Moreover, after 5 cycles via centrifugal recovery, Cat-HNFs still remained 68% of their initial activity. The detection result in human serum suggested Cat-HNFs can be a promisng candidate for the detection of H 2 O 2 in biomedical field.