Elaeagnus multiflora fruit extract inhibits melanin biosynthesis via regulation of tyrosinase gene on translational level

The goal of this study obtains basal data for application as a functional cosmetic material to induce whitening by an extract of Elaeagnus multiflora fruits. Tyrosinase activity, which plays a critical role for melanin biosynthesis to regulate skin whitening, was completely inhibited by addition of 30 mu g/ml the extract. Furthermore, tyrosine inhibitor(s) among the extract of E. multiflora fruits exhibited high temperature stability. The melanin biosynthesis to B16BL6 mouse melanoma cell was inhibited above 90% by addition of 3 mu g/ml of the extract. However, cytotoxicity was never observed to melanoma cell at high concentration of 15 mu g/ml of the extract. E. multiflora fruit extract was not involved in modulation of tyrosinase gene on transcriptional level but associated on translational level. All taken together, E. multiflora fruit extracts are able to modulate activity of tyrosinase via regulation on translation level. Therefore, these results suggest that E. multiflora fruit extract is a potential possibility of application as a whitening functional cosmetic material through repression of melanin biosynthesis.