Insect Cell Culture Using the New Brunswick ™ BioFlo ® /CelliGen ® 115 Benchtop Fermentor/Bioreactor with Spin Filter Assembly

This application report presents a simple protocol for achieving high-density culture of Spodoptera frugiperda (Sf9) cells using a New Brunswick bench-top, autoclavable stirred-tank reactor with a spin-filter assembly. Factors such as substrate concentration and metabolite buildup can be limiting for culture growth and viability at high densities. Using the spin filter in a 3.0 L vessel (2.0 L working volume) attached to the BioFlo ® /CelliGen ® 115 cabinet, a cell density of 18.24 x 10 6 cells with viability over 90 % was achieved, outperforming the batch or fedbatch process.